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puc19 based hdr donor plasmids  (Addgene inc)


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    Structured Review

    Addgene inc puc19 based hdr donor plasmids
    Puc19 Based Hdr Donor Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 2984 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc19+based+hdr+donor+plasmids/pX330-U6-Chimeric_BB-CBh-hSpCas9+(Plasmid+%2342230)/pmc09804145-296-20-17
    Average 96 stars, based on 2984 article reviews
    puc19 based hdr donor plasmids - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging
    Article Snippet: .. Towards this, PARP1 and PARP2 sgRNAs were inserted into the Bbs I site of the px330 plasmid (Addgene # 42230). pUC19 based HDR (donor) plasmids containing the left and right homology regions were constructed using PCR and NEBuilder Hifi DNA assembly (New England Biosciences, #E2621). .. The template plasmid used for this process, 3xFlag-HaloTag-EZH2 HDR - pDY053, was a gift from Thomas Cech (Addgene plasmid # 171108).

    Construct:

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging
    Article Snippet: .. Towards this, PARP1 and PARP2 sgRNAs were inserted into the Bbs I site of the px330 plasmid (Addgene # 42230). pUC19 based HDR (donor) plasmids containing the left and right homology regions were constructed using PCR and NEBuilder Hifi DNA assembly (New England Biosciences, #E2621). .. The template plasmid used for this process, 3xFlag-HaloTag-EZH2 HDR - pDY053, was a gift from Thomas Cech (Addgene plasmid # 171108).

    Polymerase Chain Reaction:

    Article Title: Dynamics of endogenous PARP1 and PARP2 during DNA damage revealed by live-cell single-molecule imaging
    Article Snippet: .. Towards this, PARP1 and PARP2 sgRNAs were inserted into the Bbs I site of the px330 plasmid (Addgene # 42230). pUC19 based HDR (donor) plasmids containing the left and right homology regions were constructed using PCR and NEBuilder Hifi DNA assembly (New England Biosciences, #E2621). .. The template plasmid used for this process, 3xFlag-HaloTag-EZH2 HDR - pDY053, was a gift from Thomas Cech (Addgene plasmid # 171108).



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    96
    Addgene inc puc19 based hdr donor plasmids
    Puc19 Based Hdr Donor Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/puc19+based+hdr+donor+plasmids/pX330-U6-Chimeric_BB-CBh-hSpCas9+(Plasmid+%2342230)/pmc09804145-296-20-17
    Average 96 stars, based on 1 article reviews
    puc19 based hdr donor plasmids - by Bioz Stars, 2026-09
    96/100 stars
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